| Item type |
文献 / Documents(1) |
| 公開日 |
2022-10-28 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.3390/ijms222313159 |
|
|
言語 |
ja |
|
|
関連名称 |
10.3390/ijms222313159 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Detection of CpG Methylation in G-Quadruplex Forming Sequences Using G-Quadruplex Ligands |
|
言語 |
en |
| 著者 |
ハセガワ, ヒジリ
ササキ, イッケイ
ツカコシ, カオリ
Ma, Yue
ナガサワ, カズオ
沼田, 周助
イノウエ, ユウキ
Kim, Yeji
イケブクロ, カズノリ
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Genomic DNA methylation is involved in many diseases and is expected to be a specific biomarker for even the pre-symptomatic diagnosis of many diseases. Thus, a rapid and inexpensive detection method is required for disease diagnosis. We have previously reported that cytosine methylation in G-quadruplex (G4)-forming oligonucleotides develops different G4 topologies. In this study, we developed a method for detecting CpG methylation in G4-forming oligonucleotides based on the structural differences between methylated and unmethylated G4 DNAs. The differences in G4 topologies due to CpG methylation can be discriminated by G4 ligands. We performed a binding assay between methylated or unmethylated G4 DNAs and G4 ligands. The binding abilities of fluorescent G4 ligands to BCL-2, HRAS1, HRAS2, VEGF G4-forming sequences were examined by fluorescence-based microtiter plate assay. The differences in fluorescence intensities between methylated and unmethylated G4 DNAs were statistically significant. In addition to fluorescence detection, the binding of G4 ligand to DNA was detected by chemiluminescence. A significant difference was also detected in chemiluminescence intensity between methylated and unmethylated DNA. This is the first study on the detection of CpG methylation in G4 structures, focusing on structural changes using G4 ligands. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
DNA methylation |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
G-quadruplex |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
G4 ligand |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
biosensor |
| 書誌情報 |
en : International Journal of Molecular Sciences
巻 22,
号 23,
p. 13159,
発行日 2021-12-06
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
14220067 |
| 出版者 |
|
|
出版者 |
MDPI |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
| EID |
|
|
識別子 |
387870 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
ijms_22_23_13159.pdf (1.63 MB)
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