| Item type |
文献 / Documents(1) |
| 公開日 |
2023-01-25 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.3390/v13102024 |
|
|
言語 |
ja |
|
|
関連名称 |
10.3390/v13102024 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Development of Antibodies against HPV-6 and HPV-11 for the Study of Laryngeal Papilloma |
|
言語 |
en |
| 著者 |
イケガミ, タロウ
キセ, ノリモト
キンジョウ, ヒデトシ
コンドウ, シュンスケ
スズキ, ミキオ
ツカハラ, ナルトシ
村上, 明一
キユナ, アサノリ
アゲナ, シンヤ
タナカ, カツノリ
ハセガワ, ナルミ
カワカミ, ジュンコ
ガナハ, アキラ
マエダ, ヒロユキ
ヒラカワ, ヒトシ
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Laryngeal papilloma (LP), which is associated with infection by human papillomavirus (HPV)-6 or -11, displays aggressive growth. The precise molecular mechanism underlying the tumorigenesis of LP has yet to be uncovered. Building on our earlier research into HPV-6, in this study, the viral gene expression of HPV-11 was investigated by quantitative PCR and DNA/RNA in situ hybridization. Additionally, newly developed antibodies against the E4 protein of HPV-6 and HPV-11 were evaluated by immunohistochemistry. The average viral load of HPV-11 in LP was 1.95 ± 0.66 × 105 copies/ng DNA, and 88% of HPV mRNA expression was found to be E4, E5a, and E5b mRNAs. According to RNA in situ hybridization, E4 and E5b mRNAs were expressed from the middle to upper part of the epithelium. E4 immunohistochemistry revealed a wide positive reaction in the upper cell layer in line with E4 mRNA expression. Other head and neck lesions with HPV-11 infection also showed a positive reaction in E4 immunohistochemistry. The distribution pattern of HPV DNA, viral mRNA, and E4 protein in LP with HPV-11 infection was quite similar to that of HPV-6. Therefore, it might be possible to apply these E4-specific antibodies in other functional studies as well as clinical applications, including targeted molecular therapies in patients with HPV-6 and HPV-11 infection. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
laryngeal papilloma |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
human papillomavirus 6 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
human papillomavirus 11 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
viral DNA and mRNA expression |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
anti-E4 antibody |
| 書誌情報 |
en : Viruses
巻 13,
号 10,
p. 2024,
発行日 2021-10-07
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
19994915 |
| 出版者 |
|
|
出版者 |
MDPI |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
| EID |
|
|
識別子 |
388411 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
virus_13_10_2024.pdf (2.77 MB)
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