| Item type |
文献 / Documents(1) |
| 公開日 |
2023-12-15 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.2152/jmi.70.464 |
|
|
言語 |
ja |
|
|
関連名称 |
10.2152/jmi.70.464 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Molecular composition of adiponectin in urine is a useful biomarker for detecting early stage of diabetic kidney disease |
|
言語 |
en |
| タイトル別表記 |
|
|
その他のタイトル |
Urinary adiponectin as a biomarker for DKD |
|
言語 |
en |
| 著者 |
カワノ, トモハル
アイバ, モトヒロ
石津, 将
森, 博康
松久, 宗英
ハシダ, セイイチ
Colpitts, Tracey
ワタナベ, トシヒロ
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
We previously developed two immune complex transfer enzyme immunoassays (ICT-EIA) to measure total adiponectin (T-AN) and high molecular weight adiponectin (H-AN) in urine and have verified their usefulness as biomarkers for diabetic kidney disease. In this study, we developed T-AN and H-AN assays using the sandwich EIA (Sand-EIA). The reactivities of Sand-EIAs were compared with ICT-EIAs by measuring size exclusion chromatography (SEC) fractions of urine and adiponectin standard. As a result, ICT-EIAs showed higher macromolecular specificity. We then analyzed the molecular profile of adiponectin in the urine of 5 patients with different eGFR stages by measuring SEC fractions of urine. The results showed that smaller adiponectin correlated relatively well with eGFR stage. Finally, because SEC is time-consuming, we investigated that the ratio of T-ANs by Sand-EIA and ICT-EIA could be a good indicator of the monomer adiponectin. The ratio was evaluated using 77 urine samples from patients with diabetes and showed a significant decrease at an earlier stage compared with other biomarkers. In conclusion, we demonstrated a new index to estimate monomer adiponectin in urine by using Sand-EIA and ICT-EIA, and urinary monomer adiponectin can be a good early indicator of deterioration of renal function in diabetic patients. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Immune complex transfer enzyme immunoassay |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Ultrasensitive immunoassay |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Urinary adiponectin |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Kidney disease |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Biomarker |
| 書誌情報 |
en : The Journal of Medical Investigation
巻 70,
号 3-4,
p. 464-470,
発行日 2023-08
|
| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13496867 |
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13431420 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11166929 |
| 出版者 |
|
|
出版者 |
Tokushima University Faculty of Medicine |
|
言語 |
en |
| EID |
|
|
識別子 |
409199 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
jmi_70_3-4_464.pdf (691 KB)
