| Item type |
文献 / Documents(1) |
| 公開日 |
2024-02-26 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1007/s13577-021-00621-0 |
|
|
言語 |
ja |
|
|
関連名称 |
10.1007/s13577-021-00621-0 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Establishment of Down’s syndrome periodontal ligament cells by transfection with SV40T-Ag and hTERT |
|
言語 |
en |
| 著者 |
アサカワ, タケヨシ
ヤマダ, アツシ
クギノ, マスミ
長谷川, 智一
ヨシムラ, ケンタロウ
ササ, キヨヒト
キノシタ, ミツヒロ
ニッタ, マサカズ
ナガタ, カリン
スギヤマ, トモミ
カミジョウ, リュウタロウ
フナツ, タカヒロ
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Down’s syndrome is one of the most common human congenital genetic diseases and affected patients have increased risk of periodontal disease. To examine involvement of the disease with periodontal disease development, we established immortalized periodontal ligament cells obtained from a Down’s syndrome patient by use of SV40T-Ag and hTERT gene transfection. Expressions of SV40T-Ag and hTERT were observed in periodontal ligament cell-derived immortalized cells established from healthy (STPDL) and Down’s syndrome patient (STPDLDS) samples. Primary cultured periodontal ligament cells obtained from a healthy subject (pPDL) had a limited number of population doublings (< 40), while STPDL and STPDLDS cells continued to grow with more than 80 population doublings. Primary cultured periodontal ligament cells obtained from the patient showed a chromosome pattern characteristic of Down’s syndrome with trisomy 21, whereas STPDLDS samples showed a large number of abnormal chromosomes in those results. Gene expression analysis revealed that expression of DSCR-1 in STPDLDS is greater than that in STPDL. These results suggest that the newly established STPDLDS cell line may be a useful tool for study of periodontal disease in Down’s syndrome patients. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Periodontal ligament cells |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Down’s syndrome |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
DSCR-1 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
SV40T-Ag |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
hTERT |
| 書誌情報 |
en : Human Cell
巻 35,
号 1,
p. 379-383,
発行日 2021-09-29
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
17490774 |
| 出版者 |
|
|
出版者 |
Japan Human Cell Society |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Springer Nature |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
| EID |
|
|
識別子 |
399244 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
hc_35_1_379.pdf (2.6 MB)
