| Item type |
文献 / Documents(1) |
| 公開日 |
2024-07-31 |
| アクセス権 |
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|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1016/j.jbc.2024.107505 |
|
|
言語 |
ja |
|
|
関連名称 |
10.1016/j.jbc.2024.107505 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
ArcS from Thermococcus kodakarensis transfers L-lysine to preQ0 nucleoside derivatives as minimum substrate RNAs |
|
言語 |
en |
| タイトル別表記 |
|
|
その他のタイトル |
Substrate RNA specificity of ArcS |
|
言語 |
en |
| 著者 |
フジタ, シュウ
スギオ, ユズル
カワムラ, タクヤ
ヤマガミ, リョウタ
オカ, ナツヒサ
平田, 章
ヨコガワ, タカシ
ホリ, ヒロユキ
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Archaeosine (G+) is an archaea-specific tRNA modification synthesized via multiple steps. In the first step, archaeosine tRNA guanine transglucosylase (ArcTGT) exchanges the G15 base in tRNA with 7-cyano-7-deazaguanine (preQ0). In Euryarchaea, preQ015 in tRNA is further modified by archaeosine synthase (ArcS). Thermococcus kodakarensis ArcS catalyzes a lysine-transfer reaction to produce preQ0-lysine (preQ0-Lys) as an intermediate. The resulting preQ0-Lys15 in tRNA is converted to G+15 by a radical S-adenosyl-L-methionine enzyme for archaeosine formation (RaSEA), which forms a complex with ArcS. Here, we focus on the substrate tRNA recognition mechanism of ArcS. Kinetic parameters of ArcS for lysine and tRNA-preQ0 were determined using a purified enzyme. RNA fragments containing preQ0 were prepared from Saccharomyces cerevisiae tRNAPhe-preQ015. ArcS transferred 14C-labeled lysine to RNA fragments. Furthermore, ArcS transferred lysine to preQ0 nucleoside and preQ0 nucleoside 5′-monophosphate. Thus, the L-shaped structure and the sequence of tRNA are not essential for the lysine-transfer reaction by ArcS. However, the presence of D-arm structure accelerates the lysine-transfer reaction. Because ArcTGT from thermophilic archaea recognizes the common D-arm structure, we expected the combination of T. kodakarensis ArcTGT and ArcS and RaSEA complex would result in the formation of preQ0-Lys15 in all tRNAs. This hypothesis was confirmed using 46 T. kodakarensis tRNA transcripts and three Haloferax volcanii tRNA transcripts. In addition, ArcTGT did not exchange the preQ0-Lys15 in tRNA with guanine or preQ0 base, showing that formation of tRNA-preQ0-Lys by ArcS plays a role in preventing the reverse reaction in G+ biosynthesis. |
|
言語 |
en |
| 書誌情報 |
en : Journal of Biological Chemistry
巻 300,
号 8,
p. 107505,
発行日 2024-06-27
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
00219258 |
| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1083351X |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA1202441X |
| 出版者 |
|
|
出版者 |
American Society for Biochemistry and Molecular Biology |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This is an open access article under the CC BY license. |
| EID |
|
|
識別子 |
409250 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
jbc_300_8_107505.pdf (3.14 MB)
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