| Item type |
文献 / Documents(1) |
| 公開日 |
2025-03-07 |
| アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.1074/jbc.M110.110718 |
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関連名称 |
10.1074/jbc.M110.110718 |
| 出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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タイトル |
Crystal Structure of Aminomethyltransferase in Complex with Dihydrolipoyl-H-Protein of the Glycine Cleavage System : IMPLICATIONS FOR RECOGNITION OF LIPOYL PROTEIN SUBSTRATE, DISEASE-RELATED MUTATIONS, AND REACTION MECHANISM |
| タイトル別表記 |
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その他のタイトル |
Crystal Structure of T.H Protein Complex of GCS |
| 著者 |
Okamura-Ikeda, Kazuko
Hosaka, Harumi
真板, 宣夫
Fujiwara, Kazuko
Yoshizawa, Akiyasu C.
Nakagawa, Atsushi
谷口, 寿章
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| 抄録 |
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内容記述 |
Aminomethyltransferase, a component of the glycine cleavage system termed T-protein, reversibly catalyzes the degradation of the aminomethyl moiety of glycine attached to the lipoate cofactor of H-protein, resulting in the production of ammonia, 5,10-methylenetetrahydrofolate, and dihydrolipoate-bearing H-protein in the presence of tetrahydrofolate. Several mutations in the human T-protein gene are known to cause nonketotic hyperglycinemia. Here, we report the crystal structure of Escherichia coli T-protein in complex with dihydrolipoate-bearing H-protein and 5-methyltetrahydrofolate, a complex mimicking the ternary complex in the reverse reaction. The structure of the complex shows a highly interacting intermolecular interface limited to a small area and the protein-bound dihydrolipoyllysine arm inserted into the active site cavity of the T-protein. Invariant Arg292 of the T-protein is essential for complex assembly. The structure also provides novel insights in understanding the disease-causing mutations, in addition to the disease-related impairment in the cofactor-enzyme interactions reported previously. Furthermore, structural and mutational analyses suggest that the reversible transfer of the methylene group between the lipoate and tetrahydrofolate should proceed through the electron relay-assisted iminium intermediate formation. |
| 書誌情報 |
en : Journal of Biological Chemistry
巻 285,
号 24,
p. 18684-18692,
発行日 2010-04-06
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| 収録物ID |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
1083351X |
| 収録物ID |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
00219258 |
| 収録物ID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA1202441X |
| 出版者 |
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出版者 |
American Society for Biochemistry and Molecular Biology |
| 出版者 |
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出版者 |
Elsevier |
| 権利情報 |
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|
権利情報 |
This is an Open Access article under the CC BY license. |
| EID |
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識別子 |
210188 |
| 言語 |
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言語 |
eng |
jbc_285_24_18684.pdf (2.6 MB)
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