| Item type |
文献 / Documents(1) |
| 公開日 |
2025-03-25 |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
関連識別子 |
https://doi.org/10.1242/dev.202079 |
|
|
関連名称 |
10.1242/dev.202079 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
An inducible germ cell ablation chicken model for high-grade germline chimeras |
| 著者 |
Chen, Yi-Chen
Saito, Daisuke
Suzuki, Takayuki
竹本, 龍也
|
| 抄録 |
|
|
内容記述 |
Chicken embryos are a powerful and widely used animal model in developmental biology studies. Since the development of CRISPR technology, gene-edited chickens have been generated by transferring primordial germ cells (PGCs) into recipients after genetic modifications. However, low inheritance caused by competition between host germ cells and the transferred cells is a common complication and greatly reduces production efficiency. Here, we generated a gene-edited chicken, in which germ cells can be ablated in a drug-dependent manner, as recipients for gene-edited PGC transfer. We used the nitroreductase/metronidazole (NTR/Mtz) system for cell ablation, in which nitroreductase produces cytotoxic alkylating agents from administered metronidazole, causing cell apoptosis. The chicken Vasa homolog (CVH) gene locus was used to drive the expression of the nitroreductase gene in a germ cell-specific manner. In addition, a fluorescent protein gene, mCherry, was also placed in the CVH locus to visualize the PGCs. We named this system ‘germ cell-specific autonomous removal induction’ (gSAMURAI). gSAMURAI chickens will be an ideal recipient to produce offspring derived from transplanted exogenous germ cells. |
| キーワード |
|
|
主題 |
Chicken primordial germ cell |
| キーワード |
|
|
主題 |
MAD7 nuclease |
| キーワード |
|
|
主題 |
Nitroreductase/metronidazole |
| キーワード |
|
|
主題 |
Cell ablation |
| 書誌情報 |
en : Development
巻 150,
号 18,
p. dev.202079,
発行日 2023-09-25
|
| 収録物ID |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
09501991 |
| 収録物ID |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
14779129 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10667805 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12024599 |
| 出版者 |
|
|
出版者 |
The Company of Biologists |
| 権利情報 |
|
|
権利情報 |
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
| EID |
|
|
識別子 |
410813 |
| 言語 |
|
|
言語 |
eng |
dev_150_18_dev202079.pdf (5.6 MB)
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