| Item type |
文献 / Documents(1) |
| 公開日 |
2025-03-28 |
| アクセス権 |
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アクセス権 |
embargoed access |
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アクセス権URI |
http://purl.org/coar/access_right/c_f1cf |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.1093/jb/mvaf013 |
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関連名称 |
10.1093/jb/mvaf013 |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| タイトル |
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|
タイトル |
Method for isolation and quantification of inositol glycan produced by glycosylinositol phosphoceramide-hydrolyzing phospholipase D in plants |
| タイトル別表記 |
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その他のタイトル |
Inositol glycan from GIPC |
| 著者 |
Islam, Md Majidul
Hasi, Rumana Yesmin
Umemura, Yuta
Tanaka, Hide-Nori
Kondo, Yudai
Ishikawa, Toshiki
Nagano, Minoru
Ali, Hanif
川上, 竜巳
(中橋)粟飯原, 睦美
田中, 保
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| 抄録 |
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内容記述 |
Glycosylinositol phosphoceramide (GIPC) is the most abundant sphingolipids in plants. Previously, we found phospholipase D (PLD) activity that hydrolyzes GIPC to phytoceramide 1-phosphate (PCerP) in plants and revealed that GIPC-PLD activity is carried out by an enzyme encoded by non-specific phospholipase C3 (NPC3) gene. In this study, we established a method for isolation and quantification of inositol glycan (InoGly), a counterpart of PCerP produced from GIPC, using TLC imaging. We confirmed that A. thaliana NPC3 protein and partially purified GIPC-PLD from cabbage produced InoGly in a similar amount to that of PCerP from purified GIPC. We applied our method to determination of InoGly present in plant tissues and found that it was present at 40–80 nmol/g (wet wt.) in cabbage leaves, radish root and broccoli stem, and increased to 80–120 nmol/g after homogenization of the tissues. Similar increases in PCerP and decreases in GIPC were observed after homogenization, indicating that InoGly and PCerP were produced from GIPC by GIPC-PLD activity in response to homogenization. We believe our method, which does not require the complicated process or large device, will contribute to a better understanding of GIPC metabolism and signaling in plants. |
| キーワード |
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|
主題 |
glycosylinositol phosphoceramide |
| キーワード |
|
|
主題 |
phospholipase D |
| キーワード |
|
|
主題 |
non-specific phospholipase C3 |
| キーワード |
|
|
主題 |
inositol glycan |
| キーワード |
|
|
主題 |
phytoceramide 1-phosphate |
| 書誌情報 |
en : The Journal of Biochemistry
発行日 2025-03-07
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| 収録物ID |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
17562651 |
| 収録物ID |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0021924X |
| 収録物ID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12096002 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00694073 |
| 出版者 |
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|
出版者 |
Oxford University Press |
| 備考 |
|
|
値 |
This is a pre-copyedited, author-produced version of an article accepted for publication in The Journal of Biochemistry following peer review. The version of record Md Majidul Islam, Rumana Yesmin Hasi, Yuta Umemura, Hide-Nori Tanaka, Yudai Kondo, Toshiki Ishikawa, Minoru Nagano, Hanif Ali, Ryushi Kawakami, Mutsumi Aihara, Tamotsu Tanaka, Method for isolation and quantification of inositol glycan produced by glycosylinositol phosphoceramide-hydrolyzing phospholipase D in plants, The Journal of Biochemistry, is available online at: https:// doi.org/10.1093/jb/mvaf013. |
| EID |
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識別子 |
416967 |
| 言語 |
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言語 |
eng |
jb.pdf (2.4 MB)
